Within 24 hours of the tornado, more than 800 police cars, 300 ambulances, 400 fire trucks, and 1,100 emergency responders arrived to assist with the tornado response. Jay Nixon also took swift action, activating the Missouri National Guard for debris removal and signing several executive orders to support Joplin’s recovery efforts. On May 23, 2011, one day after the storm, FEMA issued an amendment to offer critical emergency needs for Jasper and Newton counties, including emergency protective measures funding. “All night, we heard loud sirens, search dogs barking and people screaming while they combed through the rubble in hopes of finding their loved ones. “It was so dark when night fell,” said KY3 On Your Side reporter Ashley Reynolds, who had the initial on-air report for KY3 on May 22, 2011. “When you talked to survivors, you could feel the heartbreak they shouldered for those hurt and those who didn’t survive.” Adler says the devastation reminded him of what he witnessed in 2005 when covering Hurricane Katrina for his previous station. The first time in Joplin, I couldn’t believe it,” said KY3 News Anchor Paul Adler. “I’ve seen damage from many tornadoes throughout the Midwest. “It was extraordinarily difficult to find all the right words.” “I still cannot wrap my mind around the absolute devastation,” said KY3 News Anchor Steve Grant. I was glad to see that thing weaken, and the tornado it was producing was sporadic but no larger than EF2.”Īs survivors slowly returned from their shelter, they came back to see their community devastated by miles upon miles of tornado damage. “By the time I reached the office, the storm had turned right and was headed for Galena. “On the current track, that tornado was headed straight for Springfield,” said Hearst. NWS reports the tornado dissipated around 6:12 p.m. Generations braved the monster storm for more than half an hour before it weakened. It intensified to catastrophic levels when it officially reached Joplin city limits at 5:41 p.m. The twister formed near the junction of State Highway JJ and West 32nd Street. NWS reports the tornado initially hit at 5:34 p.m. This provided most residents with at least 17 minutes of lead time in advance of the tornado, according to an NWS Story Map. After communicating with emergency officials, the NWS Forecast Office in Springfield issued a tornado warning for Joplin and neighboring communities at 5:17 p.m. “Two thunderstorms eventually merged just west of Joplin and began producing the mega tornado.”įast-forward several hours, storm chasers and spotters reported multiple vortices just west of Joplin city limits. “At one point there were five or six storms all capable of producing, or ones that produced a tornado over in southeast Kansas,” recalls KY3 Chief Meteorologist Ron Hearst. Meteorologists tracked a supercell thunderstorm forming between southeast Kansas and southwest Missouri, though it wasn’t exactly clear what the storm system would bring into the early evening. CT, indicating conditions were favorable for tornadoes in Missouri and three neighboring states.Ĭold fronts clashed with warm fronts throughout the afternoon. Two days later, NWS issued a tornado watch around 1:30 p.m. The National Weather Service projected the potential for a severe weather system two days in advance, issuing an alert for a hazardous weather outlook on May 20, 2011. Fewer than one percent of all tornadoes reach EF5 status, while even fewer of those strike populated areas, like Joplin, according to the National Oceanic and Atmospheric Administration. The 2011 twister not only embodied characteristics of the “Tornado Alley” region but carried them out to an extent rarely seen in the Midwest.
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Though he'd be in his fifties now, little to nothing is known about his life or identity.Īfton Elaine "Star" Burton, Charles Manson's fiancée During the proceedings, she claimed the two had a son named Charles Luther Manson. Stevens was granted a divorce in 1963, while Manson was in prison once again. However, Bugliosi speculates in Helter Skelter that Manson persuaded Stevens to get married not out of love, but so that she'd be legally protected from testifying against him in court.īy Bugliosi's account, Manson was indicted in 1960 for sex trafficking-related charges after he drove Stevens and another woman to Mexico with the intention of acting as their pimp. Krajicek writes in Charles Manson: The Man Who Murdered the Sixties, "a weeping Candy," then 19, "swore her devotion to him and begged a federal judge not to lock him up." Manson was facing time for yet another crime when, as David J. The two were married in California following his release from the facility in San Pedro, and little is known about Stevens aside from her alleged previous arrests for sex work. Manson married Leona Stevens in 1959, according to court records per LAist. Leona Rae "Candy" Stevens, Manson's second wife Manson's first son died of a self-inflicted gunshot wound in 1993. She ultimately outlived all three of her children, as Jesse died of an accidental gunshot wound at age 11 in 1971, and Jed of an alleged drug overdose in 1986. Willis and White went on to have two more sons, Jesse and Jed, before their divorce in 1965. Charles Manson and Sharon Tate Movies Coming OutĬharles Manson, Jr., took the name of Willis's second husband, Jack White, becoming Jay White.Charles Manson Fathered At Least Three Children.Willis was pregnant with their child at the time of their arrival in L.A. As recounted in Helter Skelter: The True Story of the Manson Murders, Manson was arrested again by the fall of 1955, this time for driving a car he'd stolen in Ohio across state lines (to move to California with Rosalie). The two moved to Los Angeles that same year. Willis was 15 years old when she married Manson, then 20, in January of 1955. The fact that Manson, who was living with family members near Willis's hometown at the time, had already landed in several reform schools by then did nothing to dissuade her from the romance. Here's everything to know about Charles Manson's wives, as well as the woman he was briefly engaged to.īorn and raised in West Virginia, Rosalie Jean Willis was a teenaged hospital waitress when she first met Charles Manson. Well, he charmed one wife, at least-the story of his second marriage is a bit more complicated. And prior to the headline-making chapter of his life, Manson charmed two women into becoming his wives. As hard as it is to imagine now, Manson's followers fell under his sway because they found him irresistibly magnetic. Manson succeeded in doing all of this because he was a master manipulator after discovering the self-help classic How to Win Friends and Influence Peoplein prison, Manson drew upon its ideas to win and influence Manson Family members in the late 1960s. Charles Manson is a convicted killer who famously convinced others to commit monstrous acts of violence on his behalf: The story of the Manson family's murder of Sharon Tate and her friends (or rather, an alternate version of events) appears in Quentin Tarantino's Oscar-nominated Once Upon a Time.in Hollywood. Be a fan of Nikki like the millions of other players already are Join Nikki and her pals in Baby Supermarket to assist the mayor in boosting the city's economic growth. Take on a variety of adventures to gain accolades and awards and grow your business into a roaring success. It can be a great deal of fun to run a grocery chain Enter Supermarket Mania Journey, a highly addicting time management game, and get ready to be constantly alert. Manage distinctive stores through hundreds of challenging yet entertaining levels, cater to various discerning consumers, and expand your freezers and shelves to accommodate more products. 32 have a glitch, no matter how good you do. I had the same problem.You need the security guard so I re completed some of my other levels to gain more money than before and used that money to buy a security guard. Please put your hands to work, establish different supermarkets, and assist Nikki in keeping them supplied. How do u catch the thief if you do not have the security as help You MUST buy the security guard to catch the thief. Join Nikki and her pals in Baby Supermarket to assist the mayor in boosting the city's economic growth. Be a fan of Nikki like the millions of other players already are! View more supermarket mania journey games.Begin your frantic adventure right away in Super Breaker! It can be a great deal of fun to run a grocery chain! Enter Supermarket Mania® Journey, a highly addicting time management game, and get ready to be constantly alert. Join millions of players who are already fans of Nikki Head to Tinseltown with Nikki and her friends to help the Mayor strengthen the city’s economic development. Tags: Puzzles action adventure food fruit puzzle shopping simulation Running a grocery chain can be a lot of fun Dive into this highly addictive time management game Supermarket Mania® Journey and prepare to stay on your toes every single second. Mouse Touch ControlsĢ.7 stars out of 5 (1,230 plays / 3 votes) This will be a complete walkthrough for Supermarket Mania Journey on Android and iOS. For more details on the app, check the app reviews. Supermarket Mania Journey is a mobile game released on both Android and iOS (iPhone/iPad) platform. Don't bother trying to keep watching for them, just click on the guard regularly. Facebook Twitter Supermarket Mania Journey Walkthrough Guide 2023. Complete hundreds of challenging levels on the city map Satisfy a variety of demanding customers Sell dozens of products with savvy and great service Upgrade your equipment to boost revenue Take big spins to get free crystals Visit the bank daily and receive free gifts Google Play Game Services Support Game available in: English, Chinese, Traditional Chinese, French, German, Italian, Japanese, Korean, Brazilian Portuguese, Portuguese, Russian, Spanish Compatibility notes: This game performs best on high-end smartphones and tablets. Regularly click on the guard - same for the little girls on scooters. You may disable in-app purchases in your device settings. Torgs dirty tricks! While this game is absolutely free to play, you have the ability to unlock optional bonuses via in-app purchases from within the game. Tackle numerous quests to earn achievements and rewards, and grow your enterprise into a wild success. Manage unique stores through hundreds of challenging yet amusing levels, serve different types of picky customers and upgrade your shelves and freezers so that they can hold more inventory. Roll up your sleeves, open new supermarkets and help Nikki keep them stocked to the rafters. SupermarketMania2 SupermarketMania GameplayMausser GameplaySupermarket Mania 2Gameplay (PC HD) 1080p60FPSSupermarket Mania 2 is a time-management game w. Join millions of players who are already fans of Nikki! Head to Tinsel Town with Nikki and her friends to help the Mayor strengthen the citys economic development. Scroll down to see all of the Q&A, or use the box below to add your own. We have 1 questions and 5 Supermarket Mania 2 answers. How do u catch the thief if you do not have the security as help You MUST buy the security guard to catch the thief. According to Google Play Supermarket Mania Journey achieved more than 12 million installs. Start your frenzied adventure now! Running a grocery chain can be a lot of fun! Dive into this highly addictive time management game, Supermarket Mania Journey and prepare to stay on your toes every single second. Questions and Answers for Supermarket Mania 2. Android application Supermarket Mania Journey developed by G5 Entertainment is listed under category Strategy. Stream It Or Skip It: 'Finestkind' On Paramount+, A Languidly-Paced Film About Fishing, Brothers, Fathers And Sonsīradley Cooper Makes The Case For Not Having Chairs On Set: “Your Energy Dips The Minute You Sit Down In A Chair” 'Maestro' True Story: How Accurate Is Bradley Cooper's Leonard Bernstein Movie? 'Barbie' Director Greta Gerwig Shares That Ken's Faux Mink Coat Was Inspired By Her And Ryan Gosling’s Mutual And "Very Deep Love Of Sly Stallone": "He Wore Wonderful Coats" James Cameron Praises 'Barbie' Monologue To Greta Gerwig: "You Basically Sum Up Thousands Of Years Of The Female Dilemma In One Minute" Stream It Or Skip It: 'Trevor Noah: Where Was I' On Netflix, The Comedian Gets A Lot Of Applause For Addressing America's Place In The World The 10 Best Stand-Up Comedy Specials Of 2023 Movie’ on Netflix, a Perfectly Fine Family Movie Further Proving That IP is King Stream It Or Skip It: ‘The Super Mario Bros. Stream It Or Skip It: ‘Baby Shark’s Big Movie!’ on Paramount+, a Feature-Length Animated Earworm (That Might Secretly Be A Commentary On Taylor Swift?!?) Where to Watch the 'Willy Wonka' Movies Before 'Wonka' Here’s Why Mel Gibson Is Not in ‘Chicken Run: Dawn of the Nugget’ Stream It Or Skip It: ‘Designing Christmas With You’ on GAF, in Which Two Stubborn People Decorate A House For Work Before The Holidays Stream It Or Skip It: 'The Secret Gift of Christmas' on Hallmark Channel, About A Personal Shopper Who Falls For Her Client While Doing His Holiday Shopping Stream It or Skip It: 'Friends & Family Christmas' on Hallmark, Where Fake Dating Leads to Something Special for Two Women During the Holidays Stream It or Skip It: 'Merry Magic Christmas' on Lifetime, Where a Math Lady and Theater Guy Come Together to Save a Business and Find Love They have become an essential tool for businesses in China, enabling them to offer customers a seamless payment experience. Whether it’s purchasing goods or accessing online platforms, QR codes offer a quick and hassle-free way to complete transactions. QR code payments accounted for 85% of all mobile payments in China last year, highlighting their popularity. They are widely used for mobile payments and social commerce, seamlessly integrating online and offline activities. QR codes have transformed daily life in China, providing convenience and efficiency in various areas. Convenience and efficiency in various fields However, concerns about the potential misuse of personal data and accessibility issues for certain populations also exist.ĭiscover the full range of benefits and drawbacks QR codes bring to China’s digital landscape. QR codes in China offer unparalleled convenience and efficiency in various fields, from mobile payments to contact tracing. The Benefits and Drawbacks of QR Codes in China When employees and individuals were entering buildings or public spaces, their QR codes were scanned, allowing the government to track those who have been in close contact with suspected COVID-19 patients. In Shanghai, for example, a health code program has been implemented where group health QR codes were displayed at company entrances. This helped enforce rules and regulations and enabled authorities to monitor people’s movements in public areas. With a variety of local payment apps supporting QR transactions, the ease and convenience of this system extends well beyond locals to tourists as well. You’d be surprised to know that even foreigners visiting China can conveniently make use of these QR code payment systems. Scan a code, and book your ride! It doesn’t stop at transport and shopping either some street musicians accepted donations through QR codes. Moving on to public transportation, QR code-based ticketing systems are prevalent across buses, trains, and subways. Whether it’s buskers collecting money or individual sellers transacting goods – everyone has embraced this technology wholeheartedly. QR codes even rule the roost in street markets in China. It’s become an indispensable part of their operations. They use QR codes extensively – from product descriptions to mobile payment options. Customers scan these codes to access digital menus, place orders and make payments – a seamless customer experience indeed. In the restaurant industry, you’d often see QR codes adorn tabletops, menu cards, or payment counters. It’s nothing short of a revolution how these seemingly simple barcodes have transformed day-to-day transactions. Let’s talk about the fascinating usage of QR codes in offline sectors like restaurants, retail stores, and public transportation in China. Usage in offline sectors like restaurants, retail stores, and public transportation Each user has a unique QR code that can be scanned for various activities. QR codes on WeChat serve multiple purposes beyond messaging. This integration showcases how smartphones are effectively used in China, with WeChat leading the way. WeCha t, a messaging app in China, has revolutionized communication by integrating QR codes into its platform. Integration into messaging apps like WeChat From a marketing perspective, QR codes are a convenient way to drive traffic from offline to online, redirect users when online, and complete transactions more quickly. They are commonly used in China for payment transfers, connecting with other users, and linking to official pages or content. QR codes can link to a specific webpage or complete a transaction when scanned. They consist of black modules arranged in a square grid on a white background and can be read by an imaging device. QR codes have various applications, including product tracking, item identification, time tracking, document management, and marketing. However, they quickly became popular outside of the industry because they can be read quickly and store more information than standard barcodes. QR codes, or Quick Response Codes, were originally developed for the automotive industry in Japan. QR codes have become deeply integrated into everyday life in China, with their use extending beyond just scanning for information to encompass mobile payments, social media platforms, and even offline sectors like restaurants, retail stores, and public transportation. Overall, QR codes have revolutionized the way people in China interact with the world around them. QR codes can also be found on advertisements, product packaging, and restaurant menus, offering additional information and discounts. Mobile payment platforms like WeChat Pay and Alipay have integrated QR codes, making cashless transactions popular and convenient. They are used for payments, accessing information, and even tracking health status during the COVID-19 pandemic. In China, QR codes have become a part of daily life. Using your Xbox controller as a mouse, point the Edge browser to.If you don’t have the Microsoft Edge browser installed, grab it from the Microsoft Store app on your Xbox. Launch the Edge browser on your Xbox console from the “My Games & Apps” section on your Xbox’s homepage.You need a bit of prep work to download RetroArch. Install the Gamr13 App Store on Your Xbox A PC: you’ll need to use a Windows 10/11 PC to format the USB drive and transfer all of your games.Īlso read: PS4/PS5 Controller Alternatives for Those Who Prefer the Xbox One Controller 1.This way you’ll have the option of adding more games in the future. As far as storage capacity is concerned, it will largely depend on what consoles you want to emulate, with newer consoles having larger ROM file sizes. A USB drive: this is where all of your ROMs and BIOS files will be stored.You just need to be connected to the Internet. An Xbox console: the method of installing RetroArch is identical for Xbox One/One X and Xbox Series S/X.Gamr13 can be found on Twitter, Reddit, Discord and YouTube. MakeTechEasier is not affiliated with gamr13 in any way, however, we felt that it was only right to acknowledge the hard work that went into this project. In addition, because you’re playing on an Xbox, the Xbox controller works automatically with all of the cores within RetroArch, so you won’t have to waste time configuring controls for each emulator – you can get right into the game!Īll of this is possible thanks to the hard work of an independent developer known as gamr13. However, its main draw is that it allows all of your retro games to be accessed from a single app.Īlso, with RetroArch, you won’t lose access to your Live account, your games or your achievements. It has a number of features that you can tinker with. RetroArch is a frontend for a wide variety of emulators. Set a Hotkey on Your Xbox ControllerĪlso read: Retroarch for Android: The Complete Guide Why Use RetroArch on Xbox The issue was the formula is so light and undetectable on the skin I truly couldn’t identify any differentiation in texture between my skin and the foundation. I applied using my go-to Tarte the buffer™ brush ($48 at Sephora - waitlist only), swiping and buffing the product into my skin before stippling for extra foundation where needed. Initially I was a bit confused by the coverage of the foundation. Seriously, if you could bottle and sell beautiful skin then this would be it. While I’d love to hoard as much makeup as humanly possible (especially when it’s one of Charlotte Tilbury’s best-selling products ), I decided against gatekeeping the heavenly experience of applying the foundation and shared the extra tubes with a few friends to get their opinion on the stuff as well (I settled on shade 4 neutral, while they sampled 5 neutral and 3 neutral they loved it and the glow was unreal all around).Īs for my review, I was pleasantly surprised by just how accurate the product name was. So you’ll imagine my excitement when a trio of me-hued shades of Charlotte Tilbury Beautiful Skin ($65 at Charlotte Tilbury ) landed on my doorstep. I’ve been chomping at the bit to get my hands on a tube of Tilbury’s newest addition to her stable of skin-perfecting foundation formulas. When you follow a skincare routine for oily skin apply a face primer to create a barrier between your skin and makeup and thoroughly remove your makeup with a high-performance makeup remover, you can create beautiful makeup looks on oily skin while protecting your complexion.When and where can we get our mitts on the stuff?Īs you might have guessed, Tilbury’s new base-perfecting foundation is initially being released at for AU $65, but no doubt we’ll see the foundation rollout to local MECCA stores soon after (AKA the official Charlotte Tilbury retailer here in Australia).Ĭharlotte Tilbury Beautiful Skin Foundation officially launched January 4th, which means your New Year’s resolution to become the hottest version of yourself just became all that much easier. Makeup that’s rich in oils can be prone to clogging pores which can be problematic for all skin types but especially for oily skin. No – makeup won’t make your skin oilier or irritated as long as you follow a dedicated skin prep and makeup removal routine and use high-quality, breathable products that work for your skin type. I recommend Glow Toner, Charlotte’s Magic Serum Crystal Elixir and Charlotte’s Magic Water Cream as the perfect pore-refining trilogy!ĭoes wearing makeup make oily skin worse? Skincare products that are infused with ingredients like niacinamide are perfect choices for oily skin types with enlarged pores as the superpowered ingredient helps minimise and tighten the look of pores to help the complexion appear clearer. The best way to manage oily skin with enlarged pores is to reach for pore-refining skincare products that help to reduce their appearance. How can oily skin types manage enlarged pores? If you continue to experience oiliness throughout the day, reach for blotting papers that are designed to remove oil and unwanted shine, or apply a translucent setting powder that helps to mattify the look of your complexion. By consistently using skincare products for oily skin that help to train your skin to manage its natural oils, oily skin becomes easier to manage. The solution to stopping your face becoming oily starts in the morning with your skincare routine for oily skin. How Can I Stop My Face Being Oily During the Day? For oily skin, reach for a lightweight gel moisturiser like Charlotte’s Magic Water Cream and you will notice that skin feels smooth and hydrated without any of the heaviness or oiliness that you may associate with moisturiser.Īre you dehydrated darling? Discover the signs of dehydrated skin Every skin type can experience dehydration - even oily skin - and moisturising the face is an essential step in every skincare routine. Some people assume that applying moisturiser on oily skin will make the complexion feel greasy and oily, but this isn’t the case. Yes – it’s important to moisturise your oily skin to keep it feeling healthy and hydrated. Should I Moisturise If I Have Really Oily Skin? Choosing the best cleanser for oily skin allows you to target oiliness within your skincare routine – when you use a cleanser that specifically addresses oiliness, there shouldn’t be the need to wash your face more than the recommended twice a day. No – while you may feel that you want to wash your face more regularly when experiencing oiliness, the key to cleansing oily skin isn’t to wash your face more, but to use an effective, high-performance cleanser that helps to successfully balance your oils. Should you wash your face more when it’s oily? Skincare for Oily Skin: More Frequently Asked Questions Logitech H390 headset delivers enhanced audio quality for various multimedia purposes. The left-sided mic provides optimal voice capture, ensuring your voice comes through clearly to the person on the other end of the call. The adjustable headband and swivel-mounted leatherette ear cushions provide a comfortable fit, even during extended periods of wear. So, in a busy office environment or a noisy home, you can communicate clearly and precisely. It comes with a noise-cancellation technology that effectively filters background noise, allowing for crystal-clear conversations. With its USB connection, you can easily connect the headset to your computer and start using it immediately. The Logitech H390 ClearChat Comfort/USB Headset offers an exceptional audio experience for all your communication needs. They have experienced limitations in the operating distance, finding that the connection becomes unstable or drops when moving too far from the connected device. However, it is important to note that some users have reported a drawback with the Bluetooth connectivity range. User Feedback – Users have appreciated the headset’s ability to block out background noise effectively, ensuring clear and crisp voice transmission. The manufacturer provides 36 months of support for this product. With USB connectivity, pairing the headset to your computer is hassle-free, providing stable and reliable performance. The headset provides complete wireless freedom with a 33-foot operating range, ensuring movement flexibility. With up to 2 days of talk time and 85 hours of standby time on a single charge, you can stay connected without worrying about battery life. It is equipped with Bluetooth technology that can be effortlessly paired with a computer and connect to two devices simultaneously, offering versatility and flexibility in managing your calls. With a simple press, you can temporarily mute the microphone, ensuring your conversations remain private and confidential when needed. The ergonomic design and soft earmuffs with comfortable protein leather ensure a pleasant wearing experience. This headset is built for all-day comfort, allowing you to wear it for extended periods without discomfort or fatigue. It is designed with top-notch features so that you can expect optimal performance and user satisfaction. Their Trucker headset is expertly engineered to cater to the needs of drivers and call center professionals. The next product on our list is from TECKNET Store. While many find it comfortable for extended wear, a few users have reported discomfort or a tight fit, particularly for individuals with larger head sizes. However, it is important to note that some users have disagreed with the headset’s comfort. User Feedback – Users have provided overwhelmingly positive feedback for the Jabra Evolve 40 Pro Headset, praising its professional features and impressive sound quality. These remarkable headphones effectively block out high-frequency noises, such as human voices, ensuring a serene and focused environment.Īs it is equipped with Jabra Intelligent Call Transfer feature, you can effortlessly transition ongoing calls to your mobile device, ensuring uninterrupted conversations even when moving without losing clarity. This headset’s on-the-ear design and plush leather-feel cushions provide unparalleled comfort, even during extended wear. You can even manually activate it when you prefer to remain undisturbed. One standout feature of the Jabra Evolve 40 is its integrated busy light, which automatically activates during calls, alerting others that you are occupied. It is also compatible with all the most popular online voice call services. It is certified for Skype for Business, Cisco, and other leading UC platforms. It has a 3.5mm jack and a USB connector to connect it to your device or work computer. This headset is tailor-made for professionals who spend extensive hours on the phone or indulge in immersive music experiences. The first product on our list is the Jabra Evolve 40 Pro headset which is meticulously crafted to elevate your productivity and immerse you in astonishing sound quality during calls. How to Choose the Best Headphone for Call Centre?īest Headset for Call Centre Table Productīest Headset for Call Centre Reviews 1. There are numerous mechanisms enabling a cell to silence a transgene, including active epigenetic silencing of the gene or transcript through recognition of viral cis elements 17, 18, 19. Semi-random integration of genetic payloads into primary cells using lentiviruses or retroviruses often leads to a reduction (or even complete loss) of transgene expression over time 16. Transgene silencing is a major hindrance to using these tools for sophisticated cell engineering and therapy 14, 15. Biomolecular tools such as next-generation receptors 4, 5, 6, clustered regularly interspaced short palindromic repeats (CRISPR) activation (CRISPRa) and CRISPR interference (CRISPRi) 7, 8, 9, 10, and logic gates 11, 12, 13 could enhance this therapeutic modality, but their long-term expression often poses a major challenge in primary cells. Efforts to improve their functionality, reduce toxicity or move beyond blood cancer into solid tumours, ageing, autoimmunity and viral clearance have leveraged combinations of tools that increase the ability of T cells to sense, process and respond to the disease. For example, chimeric antigen receptor (CAR) T cells leverage a single engineered receptor to rewire its cytotoxicity towards cancers 2, 3. Maximizing the therapeutic potential and improving the efficacy and safety of engineered cells often require the expression of large genes or of complex gene circuits 1. CLIP offers a scalable and efficient method for manufacturing engineered primary cells. We show that CLIP enables the efficient insertion and stable expression of large payloads and of two difficult-to-express viral antigens in primary T cells at low cytotoxicity. The method, which we named CLIP (for ‘CRISPR for long-fragment integration via pseudovirus’), leverages an integrase-deficient lentivirus encoding a payload flanked by homology arms and ‘cut sites’ to insert the payload upstream and in-frame of an endogenous essential gene, followed by the delivery of a CRISPR-associated ribonucleoprotein complex via electroporation. Here we report a method for the knock-in and stable expression of a large payload and for the simultaneous knock-in of two genes at two endogenous loci. Leveraging homology-directed repair to place payloads under the control of endogenous essential genes can overcome silencing but often results in low knock-in efficiencies and cytotoxicity. Large payload insertion via retroviruses is typically semi-random and hindered by transgene silencing. The targeted insertion and stable expression of a large genetic payload in primary human cells demands methods that are robust, efficient and easy to implement. Phone tumblr, iphone cute, iPhone 6s, Marble Bible Quotes HD phone wallpaper
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